Application System

Bio-photonics

Micro manipulation System (MMS)

Cells and biomolecules can be freely handled with an optical hand.

  • Utilize laser beam to freely trap or remove cells, biomolecules, and etc. with non-contact method.
  • 1064nm near-infrared laser trapping minimizes the damage on cells and biomolecules.
  • A particle of 1μm can also be trapped.
  • Two-beam optical system enables multiple operations such as stretching the micro-object or pressing them each other.
  • Two beams can also be individually controlled.
  • Vibration Isolation Table(option) provides you high stability in operation.
MMS
Granule in a living cell is optically trapped. Granule Altering orientation of a cell with 2 beams Granule
Theory of laser tapping

Refraction of light can explain the theory of Laser trapping. Picture on the right shows laser beam shaped by an objective lens trapping a globular particle that has different index of refraction from a medium.
A photon which passes through an optical path A shifts its progressing direction due to two refractions in incidence toward and from a particle. Momentum of a photon alters before and after passing through a particle, thus a particle receives impetus of counterturn from its shift. This force is indicated Fa in the figure. When this force is integrated with full laser beam, a particle gets trapped in equilibrium point as a vector of integratedforce always heads toward the optical focal point.

An example of optical system

Micro Manipulation System consists of light-source part deriving from a laser head and beam collimator, and optical system to operate laser beam within microscopic view by leading it into microscope, both of which are held in main body. The beam from a laser head is adjusted to the objective lens in use, then, splitted into two. Each beam traps particles by condensing through an objective lens after being lead to a microscope with the identical optical path after reflecting through movable mirrors so that they can be freely moved within microscopic view.

Laser Optical tweezers

It is a means of wrapping cells or particles or etc., using radiation pressure of light occurred in laser irradiation to an object, like seizing an object with a pair of tweezers, which was first thought up by Arthur Ashkin in 1970s. Recent applications of its feature, the feasibility in trapping particles of μm order, to engineering science, medical science, biology, etc., have been reported and it made the development of this device be watched with keen interest.
Sigma Koki’s Micro Manipulation System has realized introduction of laser in optical unit by application of Opticsand Opto-mechanics produced by SIGMA KOKI which enabled space-saving and high efficiency.

Laser Optical tweezers
2laser 2beam type / Trapping Laser 2W
Part Number Equipment Configuration
MMS-1064-2000-2L/2M/2S Manual 2axis / Shutter
MMS-1064-2000-2L/1M1E/2S Motorized 2axis / Shutter
MMS-1064-2000-2L/2E/2S Manual 1axis & Motorized 1axis / Shutte
1laser 2beam type / Trapping Laser 2W
Part Number Equipment Configuration
MMS-1064-2000-1L/2M/2S Manual 2axis / Shutter
MMS-1064-2000-1L/1M1E/2S Motorized 2axis / Shutter
MMS-1064-2000-1L/2E/2S Manual 1axis & Motorized 1axis / Shutter
1laser 1beam type / Trapping Laser
Part Number Equipment Configuration
MMS-1064-2000/1M Manual
MMS-1064-2000/1M/1S Manual / Shutter
MMS-1064-2000/1E/1S Motorized / Shutter
Use in combination with Epi-Fluocescence microscopy

Introduction of trapping laser into microscopes allows continued usages of existing Camera Port or Fluorescent Lamp Port. This enables laser trapping while doing Total Internal Reflection Fluorescence Microscopy or Epi-illumination Microscopy. For customized Dichroic mirrors for different trapping laser or observation wavelength, please contact our International Sales Division.

  • All sorts of operating methods and optical systems can be selected to suit sample and research purpose.
    Supplementary attachment of a piezo actuator, etc is available to operate beams, in addition to manual or motorized control.
  • Use in combination with Motorized XY stage for microscope(optional) makes various operations possible.